Includes bibliographical references and index.

Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro

methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants