| 000 -LEADER |
|---|
| fixed length control field |
02729ckm a2200265La 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
0585403295 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| ISBN |
9780585403298 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| ISBN |
1592592112 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| ISBN |
9781592592111 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
572.86028 |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Genomic imprinting [electronic resource] : methods and protocols / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication |
Totowa, New Jersey : |
| Name of publisher |
Humana Press, |
| Year of publication |
c2002. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Number of Pages |
1 online resource (xii, 377 pages) : |
| Other physical details |
illustrations; |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Methods in molecular biology ; |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Genomic imprinting -- Laboratory manuals. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Electronic books. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Genomic Imprinting |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ward, Andrew, |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=67696 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://proxy.ohiolink.edu:9099/login?url=http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=67696 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Electronic Books |
CSU-Carig