CSU-Carig
Transcription factor protocols [electronic resource] /
| Item type | Location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Electronic Books
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E-Resource Section | E-Books | 572.8845 (Browse shelf) | Available |
Browsing Cagayan State University - Carig Library Shelves , Shelving location: E-Resource Section , Collection code: E-Books Close shelf browser
Includes bibliographical references and index.
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.
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