| 000 -LEADER |
|---|
| fixed length control field |
02359cam a2200241 a 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
0896035735 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| ISBN |
9780896035737 |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
572.8845 |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Transcription factor protocols [electronic resource] / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication |
Totowa, New Jersey : |
| Name of publisher |
Humana Press, |
| Year of publication |
©2000. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Number of Pages |
1 online resource, x, 306 pages : |
| Other physical details |
illustration ; |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Methods in molecular biology ; |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.
|
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Transcription factors -- Laboratory manuals. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Transcription Factors. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Sequence Analysis, DNA. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Transfection. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Tymms, Martin J. |
| 856 42 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://www.loc.gov/catdir/enhancements/fy0825/98032442-d.html |
| 856 41 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://www.loc.gov/catdir/enhancements/fy0825/98032442-t.html |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Electronic Books |
CSU-Carig