| 000 -LEADER |
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| fixed length control field |
04554cam a22003014a 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
0896036790 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
9780896036796 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
0585403287 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
9780585403281 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
1592592333 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| ISBN |
9781592592333 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
572.7 |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Nuclease methods and protocols[electronic resource] / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication |
Totowa, New Jersey : |
| Name of publisher |
Humana Press, |
| Year of publication |
ⓒ2001. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Number of Pages |
1 online resource (xvii, 525 pages) : |
| Other physical details |
illustration (some color) ; |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Methods in molecular biology ; |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Includes bibliographical references and index |
| 505 20 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Part 1 -- Characterizing nuclease activity -- Part II -- Inhibitors and activators of nucleases -- Part III -- Relating nuclease structure and function -- Part IV -- Nucleases in the clinic -- Part V -- Assays using nucleases
|
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Processivity of DNA repair enzymes / R.S. Lloyd -- Analysis by hplc of distributive activities and the synthetic (back) reaction of pancreatic-type ribonucleases / M.V. Nogues and C.M. Cuchillo -- Ire1p: A kinase and site-specific endoribonuclease / T.N. Gonzalez and P. Walter -- Microtiter-plate assay and related assays for nonspecific endonucleases / G. Meiss ... [et al.] -- Quantitating mrnas with relative and competitive RT-CPR / E.A. Prediger -- Expressing self-incompatibility RNases (s-RNases) in transgenic plants / B. Beecher and B.A. McClure -- Molecular cloning, tissue distribution, and chromosomal localization of the human homolog of the R2/Th/Stylar ribonuclease gene family / F. Acquati ... [et al.] -- Ribonuclease inhibitors / B.L. Pasloske -- Producing soluble recombinant RNases and assays to measure their interaction with interferon-[gamma] in vitro / C.H. Schein -- Assays for the evaluation of HIV-1 integrase inhibitors / Z. Debyser ... [et al.] -- The direction of ribonucleases H by antisense oligodeoxynucleotides / R.V. Giles and D.M. Tidd -- The 2-5a/RNase l pathway and inhibition by RNase l inhibitor (rli) / C. Bisbal ... [et al.] -- Crystallization and crystal structure determination of ribonuclease A-ribonuclease inhibitor protein complex / B. Kobe -- Methods for studying the interaction of baRNase with its inhibitor barstar / G. Schreiber -- Assaying in vitro refolding of RNases by mass spectrometry / G. Marino, P. Pucci and M. Ruoppolo -- Dissecting nucleases into their structural and functional domains. Mapping the rna-binding surface of RNase III by NMR / A. Ramos and A. Pastore -- Using electrostatics to define the active site of serratia endonuclease / K.L. Krause and M.D. Miller -- Homology modeling and simulations of nuclease structures / K.V. Soman ... [et al.] -- Methods for determining activity and specificity of DNA binding and DNA cleavage by class II restriction endonucleases / A. Jeltsch and A.M. Pingoud -- Engineered properties and assays for human DNAse i mutants / C.Q. Pan, D.V. Sinicropi and R.A. Lazarus -- Assays for human DNAse i activity in biological matrices / D.V. Sinicropi and R.A. Lazarus -- Evaluation of retroviral ribonuclease h activity / J.T. Miller, J.W. Rausch and S.F. Le Grice -- Assays for detection of RNase a superfamily ribonucleases / H.F. Rosenberg and J.B. Domachowske -- Assay for antitumor and lectin activity in RNase homologs / K. Nitta -- Isolation and enzymatic activity of angiogenin / J.F. Riordan and R. Shapiro -- Preparation and preclinical characterization of RNase-based immunofusion proteins / D.L. Newton and S.M. Rybak -- Restriction endonucleases and their uses / R.J. Williams -- Isolation and characterization of an unknown restriction endonuclease / R.J. Williams -- Gene modification with hapaxoterministic restriction enzymes. Easing the way / S.L. Berger -- Techniques to measure nucleic acid-protein binding and specificity. Nuclear extract preparations, DNAse i footprinting, and mobility shift assays / R.A. Rippe, D.A. Brenner and A. Tugores -- Analyzing the developmental expression of sigma factors with S1-nuclease mapping / J. Kormanec -- Detection and quantitation of mRNAs using ribonuclease protection assays / E.A. Prediger |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Nucleases -- Laboratory manuals. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Deoxyribonucleases. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Ribonucleases. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Endonucleases. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Schein, Catherine H |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=67687 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://proxy.ohiolink.edu:9099/login?url=http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=67687 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Electronic Books |
CSU-Carig