Normal view MARC view ISBD view

Nuclease methods and protocols[electronic resource] / - Totowa, New Jersey : Humana Press, ⓒ2001. - 1 online resource (xvii, 525 pages) : illustration (some color) ; - Methods in molecular biology ; .

Includes bibliographical references and index

Part 1 -- Characterizing nuclease activity -- Part II -- Inhibitors and activators of nucleases -- Part III -- Relating nuclease structure and function -- Part IV -- Nucleases in the clinic -- Part V -- Assays using nucleases
Processivity of DNA repair enzymes / R.S. Lloyd -- Analysis by hplc of distributive activities and the synthetic (back) reaction of pancreatic-type ribonucleases / M.V. Nogues and C.M. Cuchillo -- Ire1p: A kinase and site-specific endoribonuclease / T.N. Gonzalez and P. Walter -- Microtiter-plate assay and related assays for nonspecific endonucleases / G. Meiss ... [et al.] -- Quantitating mrnas with relative and competitive RT-CPR / E.A. Prediger -- Expressing self-incompatibility RNases (s-RNases) in transgenic plants / B. Beecher and B.A. McClure -- Molecular cloning, tissue distribution, and chromosomal localization of the human homolog of the R2/Th/Stylar ribonuclease gene family / F. Acquati ... [et al.] -- Ribonuclease inhibitors / B.L. Pasloske -- Producing soluble recombinant RNases and assays to measure their interaction with interferon-[gamma] in vitro / C.H. Schein -- Assays for the evaluation of HIV-1 integrase inhibitors / Z. Debyser ... [et al.] -- The direction of ribonucleases H by antisense oligodeoxynucleotides / R.V. Giles and D.M. Tidd -- The 2-5a/RNase l pathway and inhibition by RNase l inhibitor (rli) / C. Bisbal ... [et al.] -- Crystallization and crystal structure determination of ribonuclease A-ribonuclease inhibitor protein complex / B. Kobe -- Methods for studying the interaction of baRNase with its inhibitor barstar / G. Schreiber -- Assaying in vitro refolding of RNases by mass spectrometry / G. Marino, P. Pucci and M. Ruoppolo -- Dissecting nucleases into their structural and functional domains. Mapping the rna-binding surface of RNase III by NMR / A. Ramos and A. Pastore -- Using electrostatics to define the active site of serratia endonuclease / K.L. Krause and M.D. Miller -- Homology modeling and simulations of nuclease structures / K.V. Soman ... [et al.] -- Methods for determining activity and specificity of DNA binding and DNA cleavage by class II restriction endonucleases / A. Jeltsch and A.M. Pingoud -- Engineered properties and assays for human DNAse i mutants / C.Q. Pan, D.V. Sinicropi and R.A. Lazarus -- Assays for human DNAse i activity in biological matrices / D.V. Sinicropi and R.A. Lazarus -- Evaluation of retroviral ribonuclease h activity / J.T. Miller, J.W. Rausch and S.F. Le Grice -- Assays for detection of RNase a superfamily ribonucleases / H.F. Rosenberg and J.B. Domachowske -- Assay for antitumor and lectin activity in RNase homologs / K. Nitta -- Isolation and enzymatic activity of angiogenin / J.F. Riordan and R. Shapiro -- Preparation and preclinical characterization of RNase-based immunofusion proteins / D.L. Newton and S.M. Rybak -- Restriction endonucleases and their uses / R.J. Williams -- Isolation and characterization of an unknown restriction endonuclease / R.J. Williams -- Gene modification with hapaxoterministic restriction enzymes. Easing the way / S.L. Berger -- Techniques to measure nucleic acid-protein binding and specificity. Nuclear extract preparations, DNAse i footprinting, and mobility shift assays / R.A. Rippe, D.A. Brenner and A. Tugores -- Analyzing the developmental expression of sigma factors with S1-nuclease mapping / J. Kormanec -- Detection and quantitation of mRNAs using ribonuclease protection assays / E.A. Prediger

ISBN: 0896036790 9780896036796 0585403287 9780585403281 1592592333 9781592592333

Subjects--Topical Terms:
Nucleases -- Laboratory manuals.
Deoxyribonucleases.
Ribonucleases.
Endonucleases.

Dewey Class. No.: 572.7