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Amino acid analysis protocols [electronic resource] /

Series: Methods in molecular biology ; Published by : Humana Press, (Totowa, New Jersey :) Physical details: 1 online resource, xi, 265 pages : illustration ; ISBN: 0896036561; 9780896036567. Year: 2001
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Electronic Books Electronic Books E-Resource Section E-Books 547.75046 (Browse shelf) Available

Includes bibliographical references and index.

Amino acid analysis: an overview -- Amino acid analysis, using postcolumn ninhydrin detection, in a biotechnology laboratory -- Purification of proteins using UltraMacro spin columns or ProSorb sample preparation cartidges for amino acid analysis -- Amino acid analysis using precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate -- Amino acid analysis by high-performance liquid chromatography after derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide(Marfey's Reagent) -- The analysis of amino acids using precolumn derivatizatin, HPLC, and electrochemical detection -- Anion exchange chromatography and intergrated amperometric detectin of amino acids -- Ion-pair chromatography for identification of picomolar-order protein on a PVDF membrane -- Capillary gas chromatographic analysis of protein and nonprotein amino acids in biological samples -- Measurement of blood plasma amino acids in ultrafiltrates by high-performance liquid chromatography with automatic precolumn o-phthaldialdehyde derivatization -- Determination of amino acids in foods by reversed-phase high-performance liquid chromatography with new precolumn derivatives, butylthiocarbamyl, and benzylthiocarbamyl derivatives compared to the phenylthiocarbamyl derivative and ion exchange chromatography -- Amino acid measurement in body fluids using PITC derivatives -- Determination of proteins, phosphatidylethanolamine, and phosphatidylserine in lipid-rich materials by analysis of phenylthiocarbamyl derivatives -- Analysis of o-phosphoamino acids in biological samples by gas chromatography with flame photometric detection -- Determination of sulfur amino acids, glutahione, and related aminothiols in biological samples by gas chromatography with flame photometric detection --- Capillary electrophoretic determination of 4-hydroxyproline -- Total plasma homocysteine analysis by HPLC with SBD-F precolumn derivatization -- Determination of early glycation products by mass spectrometry and quantification of glycation mediated protein crosslinks by the incorporation of [¹⁴C]lysine into proteins.

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