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Integrin protocols [electronic resource] /

Additional authors: Howlett, Anthony.
Series: Methods in molecular biology ; Published by : Humana Press, (Totowa, New Jersey :) Physical details: 1 online resource, xi, 296 pages : illustration ; ISBN: 0896035697 ; 9780896035690. Year: 1999
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Item type Location Collection Call number Status Date due Barcode
Electronic Books Electronic Books E-Resource Section E-Books 572.68 (Browse shelf) Available

Includes bibliographical references and index.

pt. 1. Working with integrins -- Integrin-binding peptides derived from phage display libraries -- Production of rat monoclonal antibiodies specific for mouse integrins -- Biochemical analysis of integrin-mediated Shc signaling -- Assays for kinase activity -- Immunoblotting of integrins -- Application of flow cytometry in the analysis and sterile sorting of cell populations based on integrin expression -- Expression of integrin transcripts in human cancer cells -- pt. 2. Functional analysis of integrins -- Functional analysis of the [beta]₂ integrins -- expression of heterologous integrin genes -- Preparing a polyclonal antibody to mouse [beta]₁ integrin with function-blocking activity -- Integrin gene targeting -- Determination of positive and negative regulatory elements -- pt. 3. Integrin function at the cellular level -- A cell-based adhesion assay for the characterization of integrin [alpha]v[beta]₃ antagonists -- Integrins in cell migration -- Models for studying cellular invasion of basement membranes -- Methods for studying anoikis -- Use of the 10-day-old chick embryo model for studying angiogenesis -- Cell shape and integrin signaling regulate the differentiation state of mammary epithelial cells.

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