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Stress response [electronic resource] : methods and protocols /

Additional authors: Keyse, Stephen M.
Series: Methods in molecular biology ; Published by : Humana Press, (Totowa, New Jersey :) Physical details: 1 online resource,xvi, 488 pages : illustration ; ISBN: 0896036111 ; 9780896036116 . Year: 2000
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Includes bibliographical references and index

pt. 1. Detection and assay of stress-induced damage -- Identifying and counting protein modificatins triggered by nitrosative stress -- Determinatin of carbonyl groups in oxidized proteins -- Quantitation of 4-hydroxynonenal protein adducts -- Detection of oxidative stress in lymphocytes using dichlorodihydrofluorescein diacetate -- The measurement of protein degradation in response to oxidative stress -- pt. 2. Teh activation of signal transduction by cellular stress -- Analysis of the role of teh AMP-activated protein kinase in the response to cellular stress -- Detection and activation of stress-responsive tyrosine kinases -- Detection of DNA-dependent protein kinase in extracts from human and rodent cells -- pt. 3. The analysis of stress-induced gene expression -- pt. 4. Analysis of stress protein function.

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