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Molecular embryology [electronic resource] : methods and protocols /

Additional authors: Sharpe, Paul T | Mason, Ivor
Published by : Humana Press, (Totowa, New Jersey : ) Physical details: 1 online resource, xvi, 756 pages : illustration. ISBN: 0896033872. Year: 1999
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Item type Location Collection Call number Status Date due Barcode
Electronic Books Electronic Books E-Resource Section E-Books 571.8616 (Browse shelf) Available

Includes bibliographical references and index

Pt.1. The mouse embryo -- The mouse as a developmental model -- Culture of postimplantation mouse embryos -- Organ culture in the analysis of tissue interactions -- Treatment of mice with retinoids in vivo and in vitro: skeletal staining -- Cell grafting and labeling in postimplantation mouse embryos -- Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells -- Gene trapping in mouse embryonic stem cells -- Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse -- Reporter genes for the study of transcriptional regulation in transgenic mouse embryos -- Application of IacZ transgenic mice to cell lineage studies -- Mouse primordial germ cells: isolation and in vitro cultue -- Pt.2. The chicken embryo -- The avian embryo: an overview -- Chick embryos: incubation and isolation -- New culture -- Grafting Hensen's node -- Grafting of Somites -- Notochord grafts -- Transplantation of avian neural tissue -- Grafting of apical ridge and polarizing region --- Tissue recombinations in collagen gels -- Quail-chick chimeras -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo -- Pt.3. Amphibian embryo -- An overview of Xenopus development -- Mesoderm induction assays -- Experimental embryological methods for analysis of neural induction in the amphibian -- A method for generating transgenic frog embryos -- Axolotl/newt -- Pt.4. Zebrafish -- Pt.5. Nonvertebrate chordates -- Pt.6. Retroviruses -- Pt.7. Molecular techniques -- Pt.8. Microscopy and photography

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