| 000 -LEADER |
|---|
| fixed length control field |
02367cam a22002054a 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
0896036626 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
9780896036628 |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
573.8465 |
| 245 00 - TITLE STATEMENT |
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| Title |
Neuropeptide Y protocols [electronic resource] / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication |
Totowa, New Jersey : |
| Name of publisher |
Humana Press, |
| Year of publication |
©2000. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Number of Pages |
1 onlline resource, x, 246 pages : |
| Other physical details |
illustration ; |
| 490 1# - SERIES STATEMENT |
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| Series statement |
Methods in molecular biology ; |
| 500 ## - GENERAL NOTE |
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| General note |
Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
pt. 1. Cloning techniques -- Cloning neuropeptide tyrosine cDNA -- Human Y1/Y5 receptor gene cluster: isolation and characterization -- Human type 2 neuropeptide Y receptor gene: isolation and characterization -- Y4 receptor in different species: functional expression and binding -- Neuropeptide Y Y5 receptor expression -- Homology-based cloning methods: identification of the NPY Y2, Y4, and Y6 receptors -- pt. 2. Production of transgenic and knock-out models -- Developing transgenic neuropeptide Y rats -- Neuropeptide Y Y1 receptor-deficient mice: generation and characterization --Pt. 3. Antisense technology -- Antisense oligonucleotide apporach to study NPY-mediated feeding signal transduction -- A G[alpha]i RNA-antisense experssion strategy to investigate coupling of peptide YY/neuropeptide Y receptor to G[alpha]i -- NPY Y5 receptor subtype: pharmacological characterization with antisense oligodeosynucleotide screening strategy -- NPY antisense oligodeoxynucleotides to study the actions of NPY -- pt. 4. Techniques to study tissue distribution of mRNA -- Localization of Y-receptor subtype mRNAs in rat brain by digoxigenin labeled in situ hybridization -- Central Y4 receptor distribution: radioactive ribonucleotide probe in situ hybridization in vitro receptor autoradiography -- Combining non-isotopic localization of NPY mRNA with immunocytochemistry -- pt. 5. Miscellaneous techniques -- Y receptors characterized by RT-PCR: distribution in rat intestine -- Quantification of NPY mRNA by ribonuclease protection assay -- Radioligand binding studies: pharmacological profiles of cloned Y-receptor subtypes. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Neuropeptide Y -- Laboratory manuals. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Balasubramaniam, Ambikaipakan. |
| 856 42 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
http://www.loc.gov/catdir/enhancements/fy0825/00020392-d.html |
| 856 41 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
http://www.loc.gov/catdir/enhancements/fy0825/00020392-t.html |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Electronic Books |
CSU-Carig