| 000 -LEADER |
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| fixed length control field |
03182cam a22002534a 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
0896038106 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| ISBN |
9780896038103 |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
616.0797 |
| 245 00 - TITLE STATEMENT |
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| Title |
T cell [electronic resource] : development and activation / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication |
Totowa, New Jersey : |
| Name of publisher |
Humana Press, |
| Year of publication |
©2000. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Number of Pages |
1 online resource, xiv, 365 pages : |
| Other physical details |
illustration ; |
| 490 1# - SERIES STATEMENT |
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| Series statement |
Methods in molecular biology ; |
| 500 ## - GENERAL NOTE |
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| General note |
Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
pt. 1. Overview: for chapters 2-15 -- Insights into T-cell development using transgenic and knockout mice -- pt. 2. T cell development -- Isolation and characterizatin of murine early intrathymic precursor populations -- Differentiation of mouse thymocytes in fetal thymus organ culture -- Purification of immature CD4+CD8+ thymyocytes by panning with anti-CD8 antibody -- Transfection and transcription of genes in developing thymocytes -- analysis of CD4/CD8 lineage commitment by pronase treatment and reexpression assay -- Intrahymic delivery of MHC genes using recombinant adenoviruses -- Quantitative analysis of the usage of human T-cell receptor [alpha] and [beta] chain variable regions by reverse dot-blot hybridization -- Flow cytometric detection of cytoplasmic and surface CD3-£ expression in developing T cells -- Assessing apoptosis in developing T cells by flow cytometry -- Isolation of T-cells subsets by magnetic cell sorting (MACS) -- Isolation of T-cell antigens by retrovirus-mediated expression cloning -- Flow cytometric analysis of murine T lymphocytes: a practical guide -- Production and characterization of T cell hybridomas -- pt. 3. Overview: for chapters 17-29 --Something happens: a brief history of TCR signal transduction -- pt. 4. T-cell activation -- Measurement of phosphatidylinositol (PI) hydrolysis in activated T lymphocytes -- Fluorescence polarization as an early measure of T-lymphocyte stimulation -- Measurement of lymphoproliferation at the single-cell level by flow cytometry -- Digital image analysis of lymphocyte activation -- Induction of T-cell signaling by immobilized integrin ligands -- Cytotoxic T-cell adherence assay (CAA) -- Detecting ubiquitinated T-cell antigen receptor subunits by immunoblotting -- Determination of CD45 tyrosine phosphatase activity in T lymphocytes -- Measurement of protein tyrosine phosphorylation in T-cell subsets by flow cytometry -- Biochemical analysis of activated T lymphocytes: protein phosphorylation and Ras, ERK and JNK activation -- Activation of heterotrimeric GTP-binding proteins upon TCR/CD3 engagement -- Generation of human T helper 1 and T helper 2 subsets form peripheral blood-derived naive CD4+ T cells -- Transcriptional expression analysis of T-cell activation by multiplex meassenger assay (MMA). |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
T cells -- Laboratory manuals. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
T-Lymphocytes. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
T-Lymphocytes. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Immunologic Techniques. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Lymphocyte Transformation. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kearse, Kelly P. |
| 856 42 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
http://www.loc.gov/catdir/enhancements/fy0825/99025100-d.html |
| 856 41 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
http://www.loc.gov/catdir/enhancements/fy0825/99025100-t.html |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Electronic Books |
CSU-Carig