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Transforming growth factor-beta protocols [electronic resource] / - Totowa, New Jersey : Humana Press, ©2000. - 1 online resource, x, 175 pages ; illustration; - Methods in molecular biology ; 142 .

Includes bibliographical references and index.

In vitro assays for measuring TGF-[beta] growth stimulation and inhibition -- Measurement of active TGF-[beta] generated by cultured cells -- Iodination fo active TGF-[beta], TGF-[beta]-receptor crosslinking, and immunoprecipitation of TGF-[beta]-receptor complexes -- Deterction of TGF-[beta] in body fluids and tissues -- Analysis of TGF-[beta]-mediated synthesis of extracellular matrix components -- Characterizatio of SMAD phosphorylatin and SMAD-receptor interaction -- Promoter analysis of TGF-[beta] responsive genes by transient transfection and deletional/mutational analysis -- Regulation of AP-1 activity by TGF-[beta] -- Cdk pathway: cyclin-dependent kinases and cyclin-dependent kinase inhibitors -- Activation of the mitogen-activated protein kinase pathway by transforming growth factor-[beta] -- Detection of TGF-[beta] type 11 receptor hot-spot mutations: the BAT-RII assay -- Mutation detection in the TGF-[beta] receptors and smad genes: RT-PCR and sequencing -- Analysis of TGF-[beta]-inducible apoptosis.

ISBN: 0896036464 9780896036468

Subjects--Topical Terms:
Transforming growth factors-beta -- Laboratory manuals.

Dewey Class. No.: 572.65